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Siirt Fıstığı Bahçelerinde Coccinellidae Türlerinin Parazitoidi Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae)’nın DNA Barkodlama ile Tanımlanması

Year 2025, Volume: 12 Issue: 2, 276 - 284, 16.04.2025

Halil Dilmen

https://doi.org/10.30910/turkjans.1531632

Abstract

Siirt fıstığı bahçelerinde, Coccinellidae familyasından çeşitli türlerin parazitoidi olan Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae)'nın tanımlanması, etkili biyolojik kontrol stratejilerinin geliştirilmesi açısından kritik öneme sahiptir. Bu çalışma, D. coccinellae’'nin DNA barkodlama yöntemi kullanılarak kesin tanımlanmasını amaçlamıştır. Araştırma, 2023 yılı boyunca Siirt fıstığı bahçelerinden düzenli olarak toplanan coccinelid örnekler üzerinde gerçekleştirilmiştir. Çalışma sonucunda, toplanan Coccinellidae türleri arasında, toplam 11 birey içinde Hippodamia variegata örneği üzerinde D. coccinellae DNA'sı doğru bir şekilde tespit edilmiştir. Dinocampus coccinellae'nin mitokondrial COI gen bölgesi, Polimeraz Zincir Reaksiyonu (PCR) ve dizileme yöntemleri kullanılarak DNA barkodlama analizi ile incelenmiştir. PCR sonuçları ve DNA dizileme analizi, D. coccinellae'nin doğru bir şekilde tanımlandığını ve yüksek bir benzerlik oranıyla NCBI veritabanındaki referans dizilerle uyumlu olduğunu ortaya koymuştur. Dizileme sonuçları, %97.13'lük bir benzerlik oranı ve 0.0 E-değeri ile yüksek doğruluk ve güvenilirlik sağlamıştır. Filogenetik ilişkilerin belirlenmesinde Maximum Likelihood (ML) yöntemi kullanılmıştır. Filogenetik analizler, D. coccinellae’nın diğer Hymenoptera türleriyle olan akrabalık ilişkilerini net bir şekilde göstermiştir. Filogenetik ağaç, D. coccinellae’nın en yakın akrabası olarak Meteorus obfuscatus ile 0.08 genetik uzaklığa sahip olduğunu ortaya koymuş, bu da türler arasındaki akrabalık ilişkileri hakkında bilgiler sunmuştur.

Keywords

Dinocampus coccinellae Coccinellidae DNA barkodlama Parazitoid COI gen

References

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  • Al-Jalely, B. H., Wang, P., Liao, Y., & Xu, W. (2022). Identification and characterization of olfactory genes in the parasitoid wasp Diadegma semiclausum (Hellén)(Hymenoptera: Ichneumonidae). Bulletin of Entomological Research, 112(2), 187-196. https://doi.org/10.1017/S0007485321000675.
  • Antil, S., Abraham, J. S., Sripoorna, S., Maurya, S., Dagar, J., Makhija, S., ... & Toteja, R. (2023). DNA barcoding, an effective tool for species identification: a review. Molecular biology reports, 50(1), 761-775. https://doi.org/10.1007/s11033-022-08015-7
  • Baena-Bejarano, N., Reina, C., Martínez-Revelo, D. E., Medina, C. A., Tovar, E., Uribe-Soto, S., ... & Gonzalez, M. A. (2023). Taxonomic identification accuracy from BOLD and GenBank databases using over a thousand insect DNA barcodes from Colombia. Plos one, 18(4), e0277379. https://doi.org/10.1371/journal.pone.0277379
  • Berkvens, N., Moens, J., Berkvens, D., Samih, M. A., Tirry, L., & De Clercq, P. (2010). Dinocampus coccinellae as a parasitoid of the invasive ladybird Harmonia axyridis in Europe. Biological control, 53(1), 92-99. https://doi.org/10.1016/j.biocontrol.2009.11.001.
  • Boehme, P., Amendt, J., Disney, R. H. L., & Zehner, R. (2010). Molecular identification of carrion-breeding scuttle flies (Diptera: Phoridae) using COI barcodes. International Journal of Legal Medicine, 124, 577-581.
  • Bolu, H. (2004). Güneydoğu Anadolu Bölgesi antepfıstığı alanlarında bulunan avcı Coccinellidae türleri, yayılış alanları ve zararlı Agonoscena pistaciae’nın populasyon değişimi üzerine etkileri. Bitki Koruma Bülteni, 44(1-4), 69-77.
  • Ceccarelli, F. S., Sharkey, M. J., & Zaldívar-Riverón, A. (2012). Species identification in the taxonomically neglected, highly diverse, neotropical parasitoid wasp genus Notiospathius (Braconidae: Doryctinae) based on an integrative molecular and morphological approach. Molecular Phylogenetics and Evolution, 62(1), 485-495. https://doi.org/10.1016/j.ympev.2011.10.018
  • Chen, H. Y., Li, H. L., Pang, H., Zhu, C. D., & Zhang, Y. Z. (2021). Investigating the Parasitoid community associated with the invasive mealybug Phenacoccus solenopsis in Southern China. Insects, 12(4), 290. https://doi.org/10.3390/insects12040290
  • Cheng, Z., Li, Q., Deng, J., Liu, Q., & Huang, X. (2023). The devil is in the details: Problems in DNA barcoding practices indicated by systematic evaluation of insect barcodes. Frontiers in Ecology and Evolution, 11, 1149839. https://doi.org/10.3389/fevo.2023.1149839
  • Dhankhar, N., & Kumar, J. (2023). Impact of increasing pesticides and fertilizers on human health: A review. Materials Today: Proceedings. https://doi.org/10.1016/j.matpr.2023.03.766
  • Dilmen, H., & Özgökçe, M. S. (2020). Siirt ili Antep fıstığı (Siirt çeşidi) bahçelerinde Agonoscena pistaciae Burckhardt and Lauterer (Hemiptera: Psyllidae)’nın popülasyon gelişmesi. Plant Protection Bulletin, 60(3), 47-56.
  • Felsenstein, J. (1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of molecular evolution, 17, 368-376.
  • Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294–299. https://doi.org/10.16955/bitkorb.675288
  • Franjević, M., Glavaš, M., Hrašovec, B., Koletić, N., & Franjević, D. (2015). Genetic identification of new alien pest species Illinoia liriodendri and its parasitoid Areopraon silvestre in Croatia. Periodicum biologorum, 117(4), 513-517. https://doi.org/10.18054/pb.2015.117.4.3713
  • Hebert, P. D. N., Cywinska, A., Ball, S. L., & deWaard, J. R. (2003). "Biological identification through DNA barcodes." Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313-321. https://doi.org/10.1098/rspb.2002.2218
  • Huang, W., Xie, X., Huo, L., Liang, X., Wang, X., & Chen, X. (2020). An integrative DNA barcoding framework of ladybird beetles (Coleoptera: Coccinellidae). Scientific Reports, 10(1), 10063. https://doi.org/10.1038/s41598-020-66874-1
  • Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), 1547-1549.
  • Koyama, S., & Majerus, M. E. (2008). Interactions between the parasitoid wasp Dinocampus coccinellae and two species of coccinellid from Japan and Britain. From Biological Control to Invasion: The Ladybird Harmonia Axyridis as a Model Species, 253-264. https://doi.org/10.1007/978-1-4020-6939-0_17
  • Nazir, T., Khan, S., & Qiu, D. (2019). Biological control of insect pest. Pests Control and Acarology, IntechOpen. 21. https://doi.org/10.5772/intechopen.81431
  • Poolprasert, P., Senarat, S., Nak-eiam, S., & Likhitrakarn, N. (2019). Molecular taxonomic identification of predatory ladybird beetles inferred from COI Sequences (Coleoptera: Coccinellidae). Malaysian Journal of Applied Sciences, 4(2), 10-18.
  • Ranjbar, F., Jalali, M. A., Ahmadi, Z., Pons, X., Levi-Mourao, A., & Ugine, T. (2024). Comparison of the predatory impacts of indigenous and adventive ladybeetle species (Coleoptera: Coccinellidae) using a functional response approach. Journal of Plant Diseases and Protection, 131(5), 1515-1523.
  • Ricupero, M., Zepeda-Paulo, F., Cabrera, N., Biondi, A., Dai, C., Zappalà, L., ... & Lavandero, B. (2023). The geographic scope of host use by the ladybeetle parasitoid Dinocampus coccinellae. Biological Invasions, 25(9), 3009-3024. https://doi.org/10.1007/s10530-023-03090-8
  • Riddick, E. W. (2017). Identification of conditions for successful aphid control by ladybirds in greenhouses. Insects, 8(2), 38. https://doi.org/10.3390/insects8020038
  • Salehi, T., Mehrnejad, M. R., & Pashaei Rad, S. (2013). Diversity pattern of adult ladybird (Coleoptera: Coccinellidae) communities on pistachio trees in southern parts of Iran in different months. Zoology and Ecology, 23(4), 286-292. https://doi.org/10.1080/21658005.2013.838071
  • Souliotis C., Markoyiannaki‐Printziou D., Lefkaditis F., (2002). The problems and prospects of ıntegrated control of Agonoscena pistaciae Burck. and Laut. (Hom.: Sternorrhyncha) in Greece. Journal of Applied Entomology. 126(7-8), 384-388.
  • Smith, M. A., Woodley, N. E., Janzen, D. H., Hallwachs, W., & Hebert, P. D. (2006). DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proceedings of the National Academy of Sciences, 103(10), 3657-3662. https://doi.org/10.1073/pnas.051131810
  • Tiring, G., Tusun, A., Kalkan, Ç., & Satar, S. (2023). The first native pupae parasitoid of Ceratitis capitata in Türkiye: The molecular identification of Pachycrepoideus vindemmiae. International Journal of Tropical Insect Science, 43(4), 1381-1385. https://doi.org/10.1007/s42690-023-01033-3
  • Özgen, İ., & Karsavuran, Y. (2005). Antepfıstığı ağaçlarında zararlı Lepidosaphes pistaciae (Archangelskaya) (Homoptera: Diaspididae)’nin doğal düşmanlarının saptanması üzerinde araştırmalar. Turkish Journal of Entomology, 29(4), 309-316.
  • Wang, L., Wu, H., He, W., Lai, G., Li, J., Liu, S., & Zhou, Q. (2024). Diversity of Parasitoid Wasps and Comparison of Sampling Strategies in Rice Fields Using Metabarcoding. Insects, 15(4), 228. https://doi.org/10.3390/insects15040228

Identification of the Parasitoid Wasp Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae) in Pistachio Orchards Using DNA Barcoding

Year 2025, Volume: 12 Issue: 2, 276 - 284, 16.04.2025

Halil Dilmen

https://doi.org/10.30910/turkjans.1531632

Abstract

Identification of Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae), a parasitoid of various species within the Coccinellidae family, is essential for the development of effective biological control strategies in pistachio orchards. This study aimed to accurately identify D. coccinellae utilizing DNA barcoding techniques. Research was conducted throughout 2023 on coccinellid specimens collected regularly from pistachio orchards in Siirt. Among the collected Coccinellidae species, D. coccinellae DNA was successfully identified in the Hippodamia variegata specimen among a total of 11 individuals. The mitochondrial COI gene region of D. coccinellae was analyzed by DNA barcoding analysis using Polymerase Chain Reaction (PCR) and sequencing methods. PCR results and DNA sequencing confirmed the accurate identification of D. coccinellae, demonstrating high similarity with reference sequences in the NCBI database. The sequencing results exhibited high accuracy and reliability, with a similarity rate of 97.13% and an E-value of 0.0. Phylogenetic relationships were assessed using the Maximum Likelihood (ML) method. Phylogenetic analyses clearly illustrated the relationships of D. coccinellae with other Hymenoptera species. The phylogenetic tree indicated that D. coccinellae is most closely related to Meteorus obfuscatus, with a genetic distance of 0.10, providing insights into interspecies relationships.

Keywords

Dinocampus coccinellae Coccinellidae DNA barcoding Parasitoid COI gene

References

  • Abdalla, H. A., Gamal Fiteha, Y., Abdel-Salam Rashed, M., Mohamed Ali, R., Mohamed Abdel-Salam Hammad, A., Saber Mohamed Bream, A., & Magdy, M. (2022). Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes. Journal of Scientific Research in Science, 39(2), 179-193. https://doi.org/10.21608/jsrs.2022.275796.
  • Al-Jalely, B. H., Wang, P., Liao, Y., & Xu, W. (2022). Identification and characterization of olfactory genes in the parasitoid wasp Diadegma semiclausum (Hellén)(Hymenoptera: Ichneumonidae). Bulletin of Entomological Research, 112(2), 187-196. https://doi.org/10.1017/S0007485321000675.
  • Antil, S., Abraham, J. S., Sripoorna, S., Maurya, S., Dagar, J., Makhija, S., ... & Toteja, R. (2023). DNA barcoding, an effective tool for species identification: a review. Molecular biology reports, 50(1), 761-775. https://doi.org/10.1007/s11033-022-08015-7
  • Baena-Bejarano, N., Reina, C., Martínez-Revelo, D. E., Medina, C. A., Tovar, E., Uribe-Soto, S., ... & Gonzalez, M. A. (2023). Taxonomic identification accuracy from BOLD and GenBank databases using over a thousand insect DNA barcodes from Colombia. Plos one, 18(4), e0277379. https://doi.org/10.1371/journal.pone.0277379
  • Berkvens, N., Moens, J., Berkvens, D., Samih, M. A., Tirry, L., & De Clercq, P. (2010). Dinocampus coccinellae as a parasitoid of the invasive ladybird Harmonia axyridis in Europe. Biological control, 53(1), 92-99. https://doi.org/10.1016/j.biocontrol.2009.11.001.
  • Boehme, P., Amendt, J., Disney, R. H. L., & Zehner, R. (2010). Molecular identification of carrion-breeding scuttle flies (Diptera: Phoridae) using COI barcodes. International Journal of Legal Medicine, 124, 577-581.
  • Bolu, H. (2004). Güneydoğu Anadolu Bölgesi antepfıstığı alanlarında bulunan avcı Coccinellidae türleri, yayılış alanları ve zararlı Agonoscena pistaciae’nın populasyon değişimi üzerine etkileri. Bitki Koruma Bülteni, 44(1-4), 69-77.
  • Ceccarelli, F. S., Sharkey, M. J., & Zaldívar-Riverón, A. (2012). Species identification in the taxonomically neglected, highly diverse, neotropical parasitoid wasp genus Notiospathius (Braconidae: Doryctinae) based on an integrative molecular and morphological approach. Molecular Phylogenetics and Evolution, 62(1), 485-495. https://doi.org/10.1016/j.ympev.2011.10.018
  • Chen, H. Y., Li, H. L., Pang, H., Zhu, C. D., & Zhang, Y. Z. (2021). Investigating the Parasitoid community associated with the invasive mealybug Phenacoccus solenopsis in Southern China. Insects, 12(4), 290. https://doi.org/10.3390/insects12040290
  • Cheng, Z., Li, Q., Deng, J., Liu, Q., & Huang, X. (2023). The devil is in the details: Problems in DNA barcoding practices indicated by systematic evaluation of insect barcodes. Frontiers in Ecology and Evolution, 11, 1149839. https://doi.org/10.3389/fevo.2023.1149839
  • Dhankhar, N., & Kumar, J. (2023). Impact of increasing pesticides and fertilizers on human health: A review. Materials Today: Proceedings. https://doi.org/10.1016/j.matpr.2023.03.766
  • Dilmen, H., & Özgökçe, M. S. (2020). Siirt ili Antep fıstığı (Siirt çeşidi) bahçelerinde Agonoscena pistaciae Burckhardt and Lauterer (Hemiptera: Psyllidae)’nın popülasyon gelişmesi. Plant Protection Bulletin, 60(3), 47-56.
  • Felsenstein, J. (1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of molecular evolution, 17, 368-376.
  • Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294–299. https://doi.org/10.16955/bitkorb.675288
  • Franjević, M., Glavaš, M., Hrašovec, B., Koletić, N., & Franjević, D. (2015). Genetic identification of new alien pest species Illinoia liriodendri and its parasitoid Areopraon silvestre in Croatia. Periodicum biologorum, 117(4), 513-517. https://doi.org/10.18054/pb.2015.117.4.3713
  • Hebert, P. D. N., Cywinska, A., Ball, S. L., & deWaard, J. R. (2003). "Biological identification through DNA barcodes." Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313-321. https://doi.org/10.1098/rspb.2002.2218
  • Huang, W., Xie, X., Huo, L., Liang, X., Wang, X., & Chen, X. (2020). An integrative DNA barcoding framework of ladybird beetles (Coleoptera: Coccinellidae). Scientific Reports, 10(1), 10063. https://doi.org/10.1038/s41598-020-66874-1
  • Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), 1547-1549.
  • Koyama, S., & Majerus, M. E. (2008). Interactions between the parasitoid wasp Dinocampus coccinellae and two species of coccinellid from Japan and Britain. From Biological Control to Invasion: The Ladybird Harmonia Axyridis as a Model Species, 253-264. https://doi.org/10.1007/978-1-4020-6939-0_17
  • Nazir, T., Khan, S., & Qiu, D. (2019). Biological control of insect pest. Pests Control and Acarology, IntechOpen. 21. https://doi.org/10.5772/intechopen.81431
  • Poolprasert, P., Senarat, S., Nak-eiam, S., & Likhitrakarn, N. (2019). Molecular taxonomic identification of predatory ladybird beetles inferred from COI Sequences (Coleoptera: Coccinellidae). Malaysian Journal of Applied Sciences, 4(2), 10-18.
  • Ranjbar, F., Jalali, M. A., Ahmadi, Z., Pons, X., Levi-Mourao, A., & Ugine, T. (2024). Comparison of the predatory impacts of indigenous and adventive ladybeetle species (Coleoptera: Coccinellidae) using a functional response approach. Journal of Plant Diseases and Protection, 131(5), 1515-1523.
  • Ricupero, M., Zepeda-Paulo, F., Cabrera, N., Biondi, A., Dai, C., Zappalà, L., ... & Lavandero, B. (2023). The geographic scope of host use by the ladybeetle parasitoid Dinocampus coccinellae. Biological Invasions, 25(9), 3009-3024. https://doi.org/10.1007/s10530-023-03090-8
  • Riddick, E. W. (2017). Identification of conditions for successful aphid control by ladybirds in greenhouses. Insects, 8(2), 38. https://doi.org/10.3390/insects8020038
  • Salehi, T., Mehrnejad, M. R., & Pashaei Rad, S. (2013). Diversity pattern of adult ladybird (Coleoptera: Coccinellidae) communities on pistachio trees in southern parts of Iran in different months. Zoology and Ecology, 23(4), 286-292. https://doi.org/10.1080/21658005.2013.838071
  • Souliotis C., Markoyiannaki‐Printziou D., Lefkaditis F., (2002). The problems and prospects of ıntegrated control of Agonoscena pistaciae Burck. and Laut. (Hom.: Sternorrhyncha) in Greece. Journal of Applied Entomology. 126(7-8), 384-388.
  • Smith, M. A., Woodley, N. E., Janzen, D. H., Hallwachs, W., & Hebert, P. D. (2006). DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proceedings of the National Academy of Sciences, 103(10), 3657-3662. https://doi.org/10.1073/pnas.051131810
  • Tiring, G., Tusun, A., Kalkan, Ç., & Satar, S. (2023). The first native pupae parasitoid of Ceratitis capitata in Türkiye: The molecular identification of Pachycrepoideus vindemmiae. International Journal of Tropical Insect Science, 43(4), 1381-1385. https://doi.org/10.1007/s42690-023-01033-3
  • Özgen, İ., & Karsavuran, Y. (2005). Antepfıstığı ağaçlarında zararlı Lepidosaphes pistaciae (Archangelskaya) (Homoptera: Diaspididae)’nin doğal düşmanlarının saptanması üzerinde araştırmalar. Turkish Journal of Entomology, 29(4), 309-316.
  • Wang, L., Wu, H., He, W., Lai, G., Li, J., Liu, S., & Zhou, Q. (2024). Diversity of Parasitoid Wasps and Comparison of Sampling Strategies in Rice Fields Using Metabarcoding. Insects, 15(4), 228. https://doi.org/10.3390/insects15040228
There are 30 citations in total.

Details

Primary Language English
Subjects Entomology in Agriculture
Journal Section Research Article
Authors

Halil Dilmen 0000-0002-3802-9947

Publication Date April 16, 2025
Submission Date August 11, 2024
Acceptance Date February 12, 2025
Published in Issue Year 2025 Volume: 12 Issue: 2

Cite

APA Dilmen, H. (2025). Identification of the Parasitoid Wasp Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae) in Pistachio Orchards Using DNA Barcoding. Turkish Journal of Agricultural and Natural Sciences, 12(2), 276-284. https://doi.org/10.30910/turkjans.1531632
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